Regulatory

Part:BBa_K2448017

Designed by: jeremy armetta   Group: iGEM17_Evry_Paris-Saclay   (2017-10-27)


pFruB from Escherichia coli

This part is the promoter region (0.25 kb upstream) of the FruB gene of Escherichia coli K-12 (ECK2162).

pFruB is repressed by FruR transcription factor (BBa_K2448009) [1, 2].

Usage and Biology

Our team used this pFruB promoter together with the E. coli FruR transcription factor (BBa_K2448009) to build a Fructose biosensor (BBa_K2448032). The results show that pFruB behaved as predicted under and without induction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None